Anti-Mitosin Antibody (3320)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IHC-P,IHC-Fr,FACS,IP Human
SKU: 3320 Categories: , ,
Overview
Product Name Anti-Mitosin Antibody (3320)
Description Anti-Mitosin Mouse Monoclonal Antibody
Target Mitosin
Species Reactivity Human
Applications WB,ICC/IF,IHC-P,IHC-Fr,FACS,IP
Host Mouse
Clonality Monoclonal
Clone ID 14C10
Isotype IgG2a
Immunogen GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Target Name Centromere protein F
Target ID Mitosin
Uniprot ID P49454
Alternative Names CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin
Gene Name CENPF
Gene ID 1063
Accession Number NP_057427
Sequence Location Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
Biological Function Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {PubMed:12974617, PubMed:17600710, PubMed:7542657, PubMed:7651420}.
Research Areas Cancer Research
Application Images
Description CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
CENPF antibody [14C10 1D8] (3320) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Description CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis.
A. 30 ug HeLa whole cell lysate/extract (untreated)
B. 30 ug HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
5% SDS-PAGE
CENPF antibody [14C10 1D8] (3320) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Description Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# 3320; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB, a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10 ug/mL.

Immunoprecipitation: use at 1-10 ug/mL.

Immunohistochemistry: use at 10 ug/mL with frozen or paraffin-embedded sections.

Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers.
References & Data Sheet
References Allred, DC et al. (1997) Lab Invest 15A (69)
Zhu, X et al. (1995), Mol Cell Biol 15: 5017-5029.
PMID 7651420