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Anti-Secretogranin II Antibody (26101)

$496.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 200ul WB,IHC,IP Human, Rat
SKU: 26101 Categories: , ,
Overview
Product Name Anti-Secretogranin II Antibody (26101)
Description Anti-Secretogranin II Rabbit Polyclonal Antibody
Target Secretogranin II
Species Reactivity Human, Rat
Applications WB,IHC,IP
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide derived from the N-terminus of mature SgII protein: QRNQLLGKEPDLRLENVQK (Accession no. AAA36607).
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Whole Antiserum
Purification Whole antiserum
Specificity Information
Specificity This antibody recognizes human, rat, and squirrel monkey SgII.
Target Name Secretogranin-2
Target ID Secretogranin II
Uniprot ID P13521
Alternative Names Chromogranin-C, Secretogranin II, SgII [Cleaved into: Secretoneurin
Gene Name SCG2
Sequence Location Secreted. Note=Neuroendocrine and endocrine secretory granules.
Biological Function Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules. {PubMed:19357184}.
Research Areas Hormones/Proteins
Background Secretogranin II (SgII), encoded by the gene SCG2, is a member of the granin family of neuroendocrine secretory proteins. The granin family also includes chromogranin A (CgA), chromogranin B (CgB), and several other secretogranin-like proteins. Studies in rodents suggest that the full-length protein, SgII, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. SgII occurs in a variety of neoplasms arising from endocrine and neuroendocrine cells and can be a useful histological tumor marker for those cells.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C to -70°C. Avoid repeated freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use diluted 1:2,500-1:10,000. A band of ~86kDa is detected. Although the predicted mw of SgII is ~71kDa, the highly acidic nature of SgII causes it to run at a higher mw in SDS-PAGE.

Immunohistochemistry: use diluted 1:1,000.

Immunoprecipitation: use diluted 1:100-1:500.

Positive control: rat PC12 adrenal pheochromocytoma cells
References & Data Sheet
References Thompson ME et al. 1992. Prolactin granulogenesis is associated with increased secretogranin expression and aggregation in the Golgi apparatus of GHvC1 cells. Endocrinology 131: 318-326.
Vasauskas AA et al. 2011. Regulation and distribution of squirrel monkey chorionic gonadotropin and secretogranin II in the pituitary. General and Comparative Endocrinology 170: 509-513.