Anti-TRAIL (CT) Antibody (2430)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 2430 Categories: , ,
Overview
Product Name Anti-TRAIL (CT) Antibody (2430)
Description Anti-TRAIL (CT) Rabbit Polyclonal Antibody
Target TRAIL (CT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide corresponding to aa 261-277 of human TRAIL (accession no. NP_003801).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody recognizes human TRAIL.
Target Name Tumor necrosis factor ligand superfamily member 10
Target ID TRAIL (CT)
Uniprot ID P50591
Alternative Names Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD antigen CD253
Gene Name TNFSF10
Gene ID 8743
Accession Number NP_003801
Sequence Location Cell membrane, Secreted
Biological Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288). Induces apoptosis. Its activity may be modulated by binding to the dPubMed:10549288, PubMed:26457518}.
Research Areas Apoptosis
Background Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. A novel member of the TNF family has been identified and designated TRAIL (TNF- related apoptosis-inducing ligand) and Apo- 2L (Apo-2 ligand). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain-containing receptors, DR4 and DR5, have been identified as the receptor for TRAIL. Like TNF and Fas ligand, TRAIL induces apoptosis and NF- B activation in many tissues and cell types.
Application Images
Description Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 ug/mL.
Description Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 ug/mL.
Description Western blot analysis of TRAIL in human brain tissue lysate with TRAIL antibody at (A) 2.5 and (B) 5 ug/mL.
Description Immunofluorescence of TRAIL in human brain tissue with Trail antibody at 20 ug/mL. Red: Trail Antibody (2430)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Positive control: HeLa cell lysate.

Immunocytochemistry: use at 20ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their application.
References & Data Sheet