Anti-DcR2 Antibody (2419)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IHC-P,IF Human
SKU: 2419 Categories: , ,
Overview
Product Name Anti-DcR2 Antibody (2419)
Description Anti-DcR2 Rabbit Polyclonal Antibody
Target DcR2
Species Reactivity Human
Applications ELISA,WB,ICC,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 249-263 of human DcR2 precursor (accession no. Q9UBN6).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length DcR2 (34kDa).
Target Name Tumor necrosis factor receptor superfamily member 10D
Target ID DcR2
Uniprot ID Q9UBN6
Alternative Names Decoy receptor 2, DcR2, TNF-related apoptosis-inducing ligand receptor 4, TRAIL receptor 4, TRAIL-R4, TRAIL receptor with a truncated death domain, CD antigen CD264
Gene Name TNFRSF10D
Gene ID 8793
Accession Number NP_003831
Sequence Location Membrane, Single-pass type I membrane protein
Biological Function Receptor for the cytotoxic ligand TRAIL (PubMed:9430226). Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis (PubMed:9537512). Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway (PubMed:9382840, PubMed:9430226). {PubMed:9382840, PubMed:9430226, PubMed:9537512}.
Research Areas Apoptosis
Background TRAIL/Apo2L is a new member of the TNF family and induces apoptosis in a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a “decoy receptorâ€. Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL- R4, or TRUNDD. DcR2 has an extracellular TRAIL-binding domain but lacks an intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuates TRAIL- induced apoptosis.
Application Images
Description Western blot analysis of DcR2 in HeLa whole cell lysate with DcR2 antibody at 1 ug/mL.
Description Immunocytochemistry staining of HeLa cells using DcR2 antibody at 10ug/mL.
Description Immunohistochemistry of DcR2 in mouse kidney node tissue with DcR2 antibody at 2.5 ug/ml.
Description Immunofluorescence of DcR2 in mouse kidney tissue withDcR2 antibody at 20 ug/ml. Green: DcR2 Antibody (2419)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Whole cell lysate from HeLa cells.
References & Data Sheet