Anti-DAP Kinase 2 Antibody (2323)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P Human, Mouse, Rat
SKU: 2323 Categories: , ,
Overview
Product Name Anti-DAP Kinase 2 Antibody (2323)
Description Anti-DAPK2 Rabbit Polyclonal Antibody
Target DAP Kinase 2
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 356-370 of human DAPK2 (accession no. BAA88063). This sequence is identical to that of mouse.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat DAPK2 (approx. 42kDa) and does not cross- react with DAPK.
Target Name Death-associated protein kinase 2
Target ID DAP Kinase 2
Uniprot ID Q9UIK4
Alternative Names DAP kinase 2, EC 2.7.11.1, DAP-kinase-related protein 1, DRP-1
Gene Name DAPK2
Gene ID 23604
Accession Number NP_055141.2
Sequence Location Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
Biological Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421). {PubMed:17347302, PubMed:24163421, PubMed:26047703}.; Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.
Research Areas Apoptosis
Background Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is a pro- apoptotic, calcium-regulated serine/threonine kinase containing a death domain. Expression of DAPK induces cell death and suppresses oncogenic trans-formation. DAPK mediates IFN -induced apoptosis. A DAPK-related protein was recently described and designated DAPK2, or DRP-1. Expression of DAPK2 induces apoptosis. The messenger RNA for DAPK2 is expressed in multiple human tissues.
Application Images
Description Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 ug/mL.
Description Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 2ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Whole cell lysate from A431cells.
References & Data Sheet