Anti-Bonzo (NT2) Antibody (2209)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 2209 Categories: , ,
Overview
Product Name Anti-Bonzo (NT2) Antibody (2209)
Description Anti-Bonzo (NT2) Rabbit Polyclonal Antibody
Target Bonzo (NT2)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 11-25 at the N-terminus of human Bonzo. The sequence of this peptide differs from those of African green monkey and macaque by one or two amino acids, respectively.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human Bonzo (40 kD).
Target Name C-X-C chemokine receptor type 6
Target ID Bonzo (NT2)
Uniprot ID O00574
Alternative Names CXC-R6, CXCR-6, CDw186, G-protein coupled receptor STRL33, G-protein coupled receptor bonzo, CD antigen CD186
Gene Name CXCR6
Gene ID 10663
Accession Number NP_006555
Sequence Location Cell membrane; Multi-pass membrane protein.
Biological Function Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.
Research Areas Infectious Disease
Background Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) require coreceptors in addition to CD4 to infect target cells. Some G protein-coupled receptors in the chemokine receptor family, including CCR5, CXCR4, CCR3, and CCR2b,have previously been identified as HIV coreceptors. An orphan G protein- coupled receptor designated Bonzo was recently cloned and identified as a HIV- 1 and -2 and SIV coreceptor (also called STRL33 and TYMSTR). The messenger RNA for Bonzo is expressed in lymphoid tissues and activated peripheral blood lymphocytes.
Application Images
Description Western blot analysis of Bonzo in human spleen tissue lysate with Bonzo antibody at 1 ug/mL in (A) the absence or (B) the presence of blocking peptide.
Description Immunohistochemistry of Bonzo in human spleen tissue with Bonzo antibody at 20 ug/mL.
Description Immunohistochemistry of Bonzo in human spleen tissue with Bonzo antibody at 2.5 ug/ml.
Description Immunofluorescence of Bonzo in human spleen tissue with Bonzo antibody at 20 ug/ml. Green: Bonzo Antibody (2209)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution.

Positive control: Tissue lysate of human spleen.
References & Data Sheet