Anti-DFF40/CAD (I18) Antibody (2107)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human, Mouse, Rat
SKU: 2107 Categories: , ,
Overview
Product Name Anti-DFF40/CAD (I18) Antibody (2107)
Description Anti-DFF40/CAD (I18) Rabbit Polyclonal Antibody
Target DFF40/CAD (I18)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 147-164 near the center of murine CAD (accession no. NP_031885). The sequence differs from human DFF40 by two amino acids.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat DFF40/CAD (40kDa).
Target Name DNA fragmentation factor subunit β
Target ID DFF40/CAD (I18)
Uniprot ID O54788
Alternative Names EC 3.-.-.-, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, DNA fragmentation factor 40 kDa subunit, DFF-40
Gene Name Dffb
Gene ID 13368
Accession Number NP_031885
Sequence Location Cytoplasm. Nucleus.
Biological Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Research Areas Apoptosis
Background Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse Dnase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxy- ribonuclease). The human homologue of mouse CAD was more recently identified and termed CPAN, DFF40, and human CAD. DFF45/ICAD is the inhibitory protein of DFF40/CAD with which it forms complexes. Upon cleavage by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD is, which causes DNA degradation, is the hallmark of apoptotic cell death.
Application Images
Description Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.
Description Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 ug/mL.
Description Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 ug/ml.
Description Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 ug/mL. Green: DFF40 Antibody (2107)
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL

Immunocytochemistry: use at 5ug/mL.

Positive control: Whole cell lysate of K562 or Jurkat cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet