Anti-CIDE-A (CT) Antibody (2089)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Mouse
SKU: 2089 Categories: , ,
Overview
Product Name Anti-CIDE-A (CT) Antibody (2089)
Description Anti-CIDE-A (CT) Rabbit Polyclonal Antibody
Target CIDE-A (CT)
Species Reactivity Mouse
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 200-214 of mouse CIDE-A (accession no. AAC34985).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse CIDE-A (25kDa) and does not cross-react with CIDE-B.
Target Name Cell death activator CIDE-A
Target ID CIDE-A (CT)
Uniprot ID O70302
Alternative Names Cell death-inducing DFFA-like effector A
Gene Name Cidea
Gene ID 12683
Accession Number NP_031728
Sequence Location Lipid droplet. Nucleus. Note=Enriched at lipid droplet contact sites. Using a GFP-tagged construct, has been shown to localize to mitochondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (PubMed:12910269). These data could not be confirmed (PubMed:22245780, PubMed:18509062).
Biological Function Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. {PubMed:18509062, PubMed:22144693, PubMed:22245780, PubMed:9564035}.
Research Areas Apoptosis
Background DFF45-related proteins CIDE-A and CIDE-B have recently been identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.
Application Images
Description Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.
Description Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 ug/mL.
Description Immunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.
Description Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 ug/ml.
Description Immunofluorescence of CIDE-A in human brain tissue with CIDE A antibody at 20 ug/mL. Green: CIDE-A Antibody (2089)
Blue: DAPI staining
Description Immunohistochemistry of CIDE-A in human brain tissue with CIDE A antibody at 2.5 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 2ug/mL.

Immunohistochemistry:use at 5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Tissue lysate of mouse heart.
References & Data Sheet