Anti-CIDE-A (CT) Antibody (2085)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 2085 Categories: , ,
Overview
Product Name Anti-CIDE-A (CT) Antibody (2085)
Description Anti-CIDE-A (CT) Rabbit Polyclonal Antibody
Target CIDE-A (CT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 200-217 of human CIDE-A (accession no. AF041378)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human CIDE-A (23kDA) and does not cross-react with CIDE-B.
Target Name Cell death activator CIDE-A
Target ID CIDE-A (CT)
Uniprot ID O60543
Alternative Names Cell death-inducing DFFA-like effector A
Gene Name CIDEA
Gene ID 1149
Accession Number NP_001270
Sequence Location Lipid droplet, Nucleus
Biological Function Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. {PubMed:19843876}.
Research Areas Apoptosis
Background DFF45-related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis which is inhibited by DFF45. CIDE-A is expressed in many tissues
Application Images
Description Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.
Description Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 ug/mL.
Description Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5ug/mL.

Immunohistochemistry: use at 5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Human brain tissue lysate.
References & Data Sheet