Anti-CAD (CT) Antibody (2011)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Mouse
SKU: 2011 Categories: , ,
Overview
Product Name Anti-CAD (CT) Antibody (2011)
Description Anti-CAD (CT) Rabbit Polyclonal Antibody
Target CAD (CT)
Species Reactivity Mouse
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 314-329 of mouse CAD (accession no. O54788).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length mouse CAD (40kDa).
Target Name DNA fragmentation factor subunit β
Target ID CAD (CT)
Uniprot ID O54788
Alternative Names EC 3.-.-.-, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, DNA fragmentation factor 40 kDa subunit, DFF-40
Gene Name Dffb
Gene ID 13368
Accession Number NP_004393
Sequence Location Cytoplasm. Nucleus.
Biological Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Research Areas Apoptosis
Background Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (caspase activated deoxyribo- nuclease). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Application Images
Description Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution.
Description Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 1 ug/mL.
Description Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 ug/mL.
Description Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 ug/ml.
Description Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 ug/ml. Green: CAD antibody (2011)
Red: Phylloidin staining
Blue: DAPI staining
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 1ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Mouse lung or spleen tissue lysate.
References & Data Sheet