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Anti-Human von Willebrand Factor Antibody (19818)

$497.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug WB,IHC Human
SKU: 19818 Categories: , ,
Overview
Product Name Anti-Human von Willebrand Factor Antibody (19818)
Description Anti-von Willebrand Factor Mouse Monoclonal Antibody
Target Human von Willebrand Factor
Species Reactivity Human
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID VW92-3
Isotype IgG2a
Immunogen Human plasma von Willebrand Factor.
Properties
Form Lyophilized
Formulation PBS, 1% BSA, pH 7.4, lyophilized.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody specifically recognizes human von Willebrand Factor V8 Protease Fragment III (N-terminal trypsin and plasmin sensitive region). This antibody does not inhibit the binding of vWF to the vitronectin receptor. This antibody does not react with bovine vWF.
Target Name von Willebrand factor
Target ID Human von Willebrand Factor
Uniprot ID P04275
Alternative Names vWF [Cleaved into: von Willebrand antigen 2
Gene Name VWF
Sequence Location Secreted, Secreted, extracellular space, extracellular matrix
Biological Function Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Application Images
Additional Information
Handling
Storage The stock solution (2. 0mg/ml) can be stored in aliquots at - 20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored.
Dilution Instructions Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).
Application Instructions ELISA: 1-10ug/ml with vWF on the solid phase

Western Blot: 5-10ug/ml; useful under non-reducing conditions.

Immunohistochemistry: 5-10ug/ml on paraffin embedded and frozen tissue sections.
References & Data Sheet