Anti-T7 Antibody, HRP conjugate (18864P)

$522.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IHC,ICC,ELISA T7
SKU: 18864P Categories: , ,
Overview
Product Name Anti-T7 Antibody, HRP conjugate (18864P)
Description Anti-T7 HRP Rabbit Polyclonal Antibody
Target T7
Species Reactivity T7
Applications WB,IHC,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Conjugate HRP
Conjugate Ratio HRP, Molar enzyme/antibody ratio = 4:1.
Immunogen T7 (MASMTGGQQMG) conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.1% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID T7
Research Areas Epitope Tags
Background The T7 tag is an 11 amino acid peptide (MASMTGGQQMG) derived from the leader sequence of T7 bacteriophage gene10 which encodes a T7 major capsid protein. The T7 tag serves as a tag in many expression vectors, and antibodies specific for the T7 tag are useful for identifying and isolating T7-tagged proteins.
Application Images
Description Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-T7 antibody 18864P used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 minutes.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet