Anti-T7 Antibody (18864-01)

$426.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IP,ICC,ELISA T7
SKU: 18864-01 Categories: , ,
Overview
Product Name Anti-T7 Antibody (18864-01)
Description Anti-T7 Rabbit Polyclonal Antibody
Target T7
Species Reactivity T7
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen T7 (MASMTGGQQMG) conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID T7
Research Areas Epitope Tags
Background The T7 tag is an 11 amino acid peptide (MASMTGGQQMG) derived from the leader sequence of T7 bacteriophage gene10 which encodes a T7 major capsid protein. The T7 tag serves as a tag in many expression vectors, and antibodies specific for the T7 tag are useful for identifying and isolating T7-tagged proteins.
Application Images
Description Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody 18864 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet