Awaiting product image

Anti-Glutathione-S-Transferase (GST) Antibody (18842-01)

$271.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug WB,IP,ICC,ELISA Glutathione-S-Transferase (Gst)
SKU: 18842-01 Categories: , ,
Overview
Product Name Anti-Glutathione-S-Transferase (GST) Antibody (18842-01)
Description Anti-Glutathione-S-Transferase (GST) Goat Polyclonal Antibody
Target Glutathione-S-Transferase (GST)
Species Reactivity Glutathione-S-Transferase (Gst)
Applications WB,IP,ICC,ELISA
Host Goat
Clonality Polyclonal
Immunogen Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by GST affinity chromatography
Specificity Information
Target ID Glutathione-S-Transferase (GST)
Research Areas Epitope Tags
Background A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
References & Data Sheet