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Anti-ECS Antibody, Agarose immobilized (18830A)

$506.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug IP Ecs
SKU: 18830A Categories: , ,
Overview
Product Name Anti-ECS Antibody, Agarose immobilized (18830A)
Description Anti-Agarose Immobilized Goat ECS Goat Polyclonal Antibody
Target ECS, Agarose immobilized
Species Reactivity Ecs
Applications IP
Host Goat
Clonality Polyclonal
Isotype Whole IgG
Conjugate Agarose
Immunogen DDDDK conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID ECS, Agarose immobilized
Uniprot ID P98073
Gene ID 5651
CAS Number 9014-74-8
Sequence Location Membrane, Single-pass type II membrane protein
Biological Function Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
Research Areas Epitope Tags
Background Enterokinase, also known as enteropeptidase, is a serine protease that catalyzes the hydrolysis of peptide bonds in proteins but, unlike other kinases, does not catalyze transfer of phosphate groups. Enterokinase cleaves proteins following a lysine at an enterokinase cleavage site, or ECS (Asp-Asp-Asp-Asp-Lys). In vivo, this cleavage results in trypsin-dependent activation of other pancreatic zymogens, such as chymotrypsinogen, proelastase, procarboxypeptidase and prolipase in the lumen of the gut.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoprecipitation: use 15-25ul of gel slurry per 0.1-1mg of protein extract or lysate.

These are recommended concentrations.

End users should determine optimal concentrations for their applications.
References & Data Sheet