Anti-6 His Antibody (18812-01)

$426.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug WB,IP,ICC,ELISA 6 His
SKU: 18812-01 Categories: , ,
Overview
Product Name Anti-6 His Antibody (18812-01)
Description Anti-6 His Goat Polyclonal Antibody
Target 6 His
Species Reactivity 6 His
Applications WB,IP,ICC,ELISA
Host Goat
Clonality Polyclonal
Immunogen 6-His (HHHHHH) conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (PBS), pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Target ID 6 His
Research Areas Epitope Tags
Background A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Application Images
Description Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-6-His antibody 18812 used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 minutes.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:5,000.

ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use at 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet