Anti-Rhinovirus 16 Antibody (18758)

$404.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC Rhinovirus 16
SKU: 18758 Categories: , , ,
Overview
Product Name Anti-Rhinovirus 16 Antibody (18758)
Description Anti-Rhinovirus 16 Mouse Monoclonal Antibody
Target Rhinovirus 16
Species Reactivity Rhinovirus 16
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID R16-7
Isotype IgG2b
Immunogen Purified HRV16.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This non-neutralizing antibody recognizes capsid protein VP2 (mw ~30kDa) of HRV16, HRV1A, and HRV39 and VP2 precursors VP0 (mw ~37kDa) and P1 (mw ~90kDa).
Target ID Rhinovirus 16
Research Areas Infectious Disease
Background Picornaviruses are small, non-enveloped RNA viruses with an icosahedral capsid and a single strand, plus-sense RNA genome. The genome encodes a single polyprotein that is proteolytically processed by viral proteases into structural and non-structural proteins. The family of picornaviruses includes numerous human and animal viruses including more than 100 s of human rhinoviruses (HRV). HRV infections are characteristic upper airway infections (the main cause of the common cold), and they provoke significant lower airway symptoms for patients with asthma, cystic fibrosis, or chronic obstructive pulmonary disease.
Application Images
Description Immunoblotting: use at 2-5ug/ml. Lysates of HeLa cells infected with RV s 1A, 2, 14, 16 or 49 and blotted with #18758.
Description Immunohistochemistry: use at 1-10ug/ml. IHC staining for viral capsid protein with #18758: (A) Uninfected HeLa cells, (B) HRV16-infected HeLa cells, (C) Negative bronchial biopsy section, (D) Positive bronchial biopsy section.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 2-5ug/mL.

Immunohistochemistry: use at 1-10ug/mL.

These are recommended concentrations;

Endusers should determine optimal concentrations for their applications.
References & Data Sheet
References Mosser AG et al. 2002 J Infect Dis 185: 734. Mosser AG et al. 2005 Am J Respir Crit Care Med 171: 645. Jurgeit A et a. 2010 Virology J 7: 264. Chattoraj SS et al. 2011 Infect Immun 79: 4131.