Awaiting product image

Anti-E. coli K99 pili Mouse Monoclonal Antibody – Library Pack (184101)

$548.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 4 x 100ug ELISA K99+ E. Coli
SKU: 184101 Categories: , , ,
Overview
Product Name Anti-E. coli K99 pili Mouse Monoclonal Antibody - Library Pack (184101)
Description Anti-E. coli K99 pili Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

18401 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9911-201.3 - Isotype IgG1

18402 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9912-349.2 - Isotype IgG1

18403 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9912-418.2 - Isotype IgG1

18404 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9912-444.5 - Isotype IgG1

Target E. coli K99 pili
Species Reactivity K99+ E. Coli
Applications ELISA
Host Mouse
Clonality Monoclonal
Immunogen Purified E. coli K99 (B44) pili.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity These antibodies recognize K99 E. coli. They do not cross-react with K99- negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
Target ID E. coli K99 pili
Background Bovine enteric colibacillosis is an economically important disease in neonatal calves caused by enterotoxigenic E. coli (ETEC) which adhere to intestinal cells using pili. Most ETEC isolated from cattle use K99 pili to bind to enterocytes of the small intestine. K99 pili are primarily composed of a repeating 18.2kDa major subunit which is responsible for binding to host glycoprotein receptor, N-glycolylneuraminic acid-GM3. A 7.1kb operon of eight genes on a plasmid encode the pili and other associated proteins responsible for regulation, transport, and assembly of K99 pili.
Application Images
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies have been qualified for use in ELISA to detect K99+ E. coli.
References & Data Sheet