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Anti-Lipoteichoic acid Antibody (15711)

$474.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 500ug ELISA,Opsonization Gram-Positive Bacteria
SKU: 15711 Categories: , ,
Overview
Product Name Anti-Lipoteichoic acid Antibody (15711)
Description Anti-Lipoteichoic Acid Mouse Monoclonal Antibody
Target Lipoteichoic acid
Species Reactivity Gram-Positive Bacteria
Applications ELISA,Opsonization
Host Mouse
Clonality Monoclonal
Clone ID 15711
Isotype IgG1
Immunogen Staphylococcus epidermidis, Hay strain (ATCC #55133).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody reacts with lipoteichoic acid of Staph epidermidis, Hay strain, as well as clinical strains of Staph. epidermidis (types I, II, and III), Staph. aureus strains 5 and 8, Strep. pyogenes, Strep. fecaelis, and Strep. mutans. It does not react with peptido- glycan of Staph. aureus or peptidoglycan- rhamnose, nor does it react with pneumococcal polysaccharides. This antibody does not cross-react with E. coli or H. influenzae type B.
Target ID Lipoteichoic acid
Research Areas Infectious Disease
Background Lipoteichoic acid (LTA) is the major proinflammatory structure present within the cell wall layer of most gram-positive bacteria. It plays an important role in the initiation and progression of bacterial infection, inflammation, and septic shock. LTA induces several cytokines in vivo, and LTA and peptidoglycan (PepG) synergize to cause the induction of nitric oxide formation which can lead to multiple organ failure. Since LTA is also found in the cell walls of non-pathogenic gram-positive bacteria, it has been suggested that the structure of LTA , and its ability to synergize with PepG, determines the ability of a particular bacterium to cause septic shock.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: use at 0.1-1.0 ug/ml (optimized for LTA on solid phase).

Opsonization assay: use at 80-160 ug/ml (optimized for Staph. epidermidis, Hay strain).
References & Data Sheet