Anti-Heme Oxygenase-1 Antibody (13062)

$346.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Human, Mouse, Rat
SKU: 13062 Categories: , ,
Overview
Product Name Anti-Heme Oxygenase-1 Antibody (13062)
Description Anti-Heme Oxygenase-1 Mouse Monoclonal Antibody
Target Heme Oxygenase-1
Species Reactivity Human, Mouse, Rat
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 6B8-2F2
Isotype IgG1
Immunogen His-tagged rat heme-oxygenase-1 (HO-1)
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat HO-1. It does not cross-react with HO-2.
Target Name Heme oxygenase 1
Target ID Heme Oxygenase-1
Uniprot ID P06762
Alternative Names HO-1, EC 1.14.14.18, HSP32
Gene Name Hmox1
Accession Number NP_036712.1
Sequence Location Microsome. Endoplasmic reticulum membrane, Peripheral membrane protein; Cytoplasmic side.
Biological Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Research Areas Enzymes
Background Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.
Application Images
Description Immunoblotting: confirmed with antibody at 1.0-2.0ug/ml and 20ug of rat kidney lysate. A band of ~31kDa is detected. Positive control: Rat kidney lysate.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: confirmed with antibody at 1-10ug/ml binding to 5ug/ml HO-1 on the solid phase.

Immunoblotting: confirmed with antibody at 1.0-2.0ug/ml and 20ug of rat kidney lysate. A band of ~31kDa is detected.

Positive control: Rat kidney lysate.

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.
References & Data Sheet