Anti-Cytochrome P450 Antibody (13006-50)

$335.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 50ug WB Human, Mouse, Rat, Rabbit, Guinea Pig, Monkey, Canine
SKU: 13006-50 Categories: , ,
Overview
Product Name Anti-Cytochrome P450 Antibody (13006-50)
Description Anti-Cytochrome P450 Rabbit Polyclonal Antibody
Target Cytochrome P450
Species Reactivity Human, Mouse, Rat, Rabbit, Guinea Pig, Monkey, Canine
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Native rat cytochrome P450.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by protein A affinity chromatography
Specificity Information
Target ID Cytochrome P450
Research Areas Enzymes
Background Cytochrome p450 is a highly conserved and diverse family of heme-containing proteins that function in the metabolism of numerous endogenous and exogenous substrates.The human genome encodes 57 p450 genes divided among 18 families which together account for the diversity of substrates metabolized. p450 is the primary conjugating enzyme in phase I metabolism and detoxification of endogenous and exogenous compounds and are primarily localized to the inner membranes of mitochondria and the ER. Eukaryotic microsomal cytochrome p450 enzymes receive electrons from the FAD- and FMN-containing enzyme NADPH:cytochrome p450 reductase.
Application Images
Description Detection of cytochrome P450 in human liver microsomes (Lane 2), rat liver microsomes (Lane 3), mouse liver microsomes (Lane 4), rabbit liver microsomes (Lane 5).
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: Use at 1-5ug/mL. A band of ~50-55kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their application.
References & Data Sheet