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Anti-Ku80 Antibody (12527)

$384.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB Human, Mouse, Rat, Hamster
SKU: 12527 Categories: , ,
Overview
Product Name Anti-Ku80 Antibody (12527)
Description Anti-Ku80 Rabbit Polyclonal Antibody
Target Ku80
Species Reactivity Human, Mouse, Rat, Hamster
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 419-440 (LVYVQLPFMEDLRQYMFSSLKN) of Ku80 conjugated to KLH at the N- terminus.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, rat, and hamster Ku80.
Target Name X-ray repair cross-complementing protein 5
Target ID Ku80
Uniprot ID P13010
Alternative Names EC 3.6.4.-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5
Gene Name XRCC5
Accession Number NP_066964
Sequence Location Nucleus, Nucleus, nucleolus, Chromosome
Biological Function Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912). Required for double-strand break repair and V(D)J rPubMed:11493912, PubMed:12145306, PubMed:20383123, PubMed:28712728, PubMed:32103174, PubMed:7957065, PubMed:8621488}.
Research Areas DNA
Background Telomere length maintenance, essential to chromosome stability and genome integrity, is regulated by telomerase and telomere-associated factors. The Ku proteins are eukaryotic nuclear proteins involved in DNA repair and telomere maintenance. They exist as a heterodimer of 70 and 80 kDa subunits (Ku 70 and Ku 80). Ku heterodimers bind to DNA ends at telomeres and at double-strand breaks where they interact with DNA-dependent protein kinase (DNA PK) and participate in targeting of DNA PK to DNA. The Ku proteins may participate in DNA repair and replication, cell signaling, proliferation, transcriptional activation, and apoptosis.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution. A band of approximately 80 kDa is detected.

Positive control: A431 and AR42J whole cell lysates.
References & Data Sheet
References Park SJ et al. (2004) J Biol Chem 279:6046-55.