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Anti-Thyroglobulin Mouse Monoclonal Antibody – Library Pack (121101)

$531.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 4 x 100ug ELISA Human
SKU: 121101 Categories: , , ,
Overview
Product Name Anti-Thyroglobulin Mouse Monoclonal Antibody - Library Pack (121101)
Description Anti-Thyroglobulin Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

12101 - Anti-Thyroglobulin Mouse Monoclonal Antibody - Clone TG60 - Isotype IgG1

12102 - Anti-Thyroglobulin Mouse Monoclonal Antibody - Clone TG62 - Isotype IgG1

12103 - Anti-Thyroglobulin Mouse Monoclonal Antibody - Clone TG8.1 - Isotype IgM

12104 - Anti-Thyroglobulin Mouse Monoclonal Antibody - Clone TG9.1 - Isotype IgG1

Target Thyroglobulin
Species Reactivity Human
Applications ELISA
Host Mouse
Clonality Monoclonal
Immunogen Purified human thyroglobulin.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Specificity Information
Specificity 12102 is T4-specific and 12104 is T3-specific. 12101 and 12103 recognize both T3 and T4.
Target Name Thyroglobulin
Target ID Thyroglobulin
Uniprot ID P01266
Gene Name TG
Gene ID 188450
Sequence Location Secreted
Biological Function Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed:32025030, PubMed:17532758). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed:32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed:32025030). One dimer produces 7 thyroid hormone molecules (PubMed:32025030). {PubMed:17532758, PubMed:32025030}.
Research Areas Hormones/Proteins
Background Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by the follicular cells of the thyroid. Thyroglobulin accounts for approximately half of the protein content of the thyroid gland. Human TG is a homodimer of subunits each containing 2768 amino acids as synthesized (a short signal peptide may be removed from the N-terminus in the mature protein). Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Application Images
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies may be used in ELISA to detect and quantitate thyroglobulin.
References & Data Sheet
References Takeyama M et al. (2012) Biochemistry 51: 820-828.Nogami K et al. (2005) Blood 105: 4362-4368.Fay PJ et al. (2001) J Biol Chem 276: 12434-12439.Fay PJ (1988) Arch Biochem Biophys 262: 525-531. Fay PJ et al. (1986) Biochim Biophys Acta 87: 268-278.