Anti-TARPγ2/4/8Stargazin Antibody (11572)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Mouse, Rat
SKU: 11572 Categories: , ,
Overview
Product Name Anti-TARPγ2/4/8Stargazin Antibody (11572)
Description Anti-TaARP2/4/8/Stargazin Mouse Monoclonal Antibody
Target TARPγ2/4/8Stargazin
Species Reactivity Mouse, Rat
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S245-36
Isotype IgG2a
Immunogen Fusion protein corresponding to aa 203-323 (cytoplasmic C-terminus) of rat TARPg2 (accession no. Q71RJ2).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse and rat TARPg2, TARPg4, and TARPg8. It does not cross-react with TARPg3
Target Name Voltage-dependent calcium channel γ-2 subunit
Target ID TARPγ2/4/8Stargazin
Uniprot ID Q71RJ2
Alternative Names Neuronal voltage-gated calcium channel γ-2 subunit, Stargazin, Transmembrane AMPAR regulatory protein γ-2, TARP γ-2
Gene Name Cacng2
Accession Number NP_445803.1
Sequence Location Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome
Biological Function Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. {PubMed:17880894, PubMed:18817736, PubMed:19234459, PubMed:19265014, PubMed:20805473}.
Research Areas Ion Channels
Background Stargazin belongs to a group of voltage- gated calcium channels that are present in most excitable cells. It appears to stabilize calcium channels in an inactivated (closed) state. Voltage-gated calcium channels exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
Application Images
Description Western Blot analysis of Rat brain lysates showing detection of Stargazin Calcium Channel protein using Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody, Clone S245-36 (11572). Primary Antibody: Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody (11572) at 1:100, 1:250, 1:500, and 1:1000.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (11572). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (11572) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone S245-36 (11572). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody (11572) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody. (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. Predicted molecular weight is 35-55kDa.

Positive control: Rat brain lysate.

These are recommended concentrations. User should determine optimal concentrations for their application.
References & Data Sheet