Anti-NALCN Antibody (11568)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Mouse, Rat
SKU: 11568 Categories: , ,
Overview
Product Name Anti-NALCN Antibody (11568)
Description Anti-NALCN Mouse Monoclonal Antibody
Target NALCN
Species Reactivity Mouse, Rat
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S185-7
Isotype IgG1
Immunogen Fusion protein corresponding to aa 1659-1738 (cytoplasmic C- terminus) of rat NALCN (accession no.Q6Q760).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse and rat NALCN.
Target Name Sodium leak channel NALCN
Target ID NALCN
Uniprot ID Q6Q760
Alternative Names Four domain-type voltage-gated ion channelα-1 subunit, Rb21-channel, Sodium leak channel non-selective protein, Voltage gated channel-like protein 1
Gene Name Nalcn
Accession Number NP_705894.1
Sequence Location Cell membrane, Multi-pass membrane protein
Biological Function Voltage-gated ion channel responsible for the resting Na(+) permeability that controls neuronal excitability. NALCN channel functions as a multi-protein complex, which consists at least of NALCN, NALF1, UNC79 and UNC80. NALCN is the voltage-sensing, pore-forming subunit of the NALCN channel complex. NALCN channel complex is constitutively active and conducts monovalent cations but is blocked by physiological concentrations of extracellular divalent cations (By similarity). In addition to its role in regulating neuronal excitability, is required for normal respiratory rhythm, systemic osmoregulation by controlling the serum sodium concentration and in the regulation of the intestinal pace-making activity in the interstitial cells of Cajal. NALCN channel is also activated by neuropeptides such as neurotensin and substance P (SP) through a SRC family kinases-dependent pathway. In addition, NALCN activity is enhanced/modulated by several GPCRs, such as CHRM3 (By similarity). {UniProtKB:Q8BXR5, UniProtKB:Q8IZF0}.
Research Areas Ion Channels
Background NALCN (sodium leak channel non- selective protein) is a 1,738 amino acid multi-pass membrane protein in the cation-nonselective channel family. NALCN is highly conserved in mammalian species and is widely expressed in the central nervous system. NALCN is a voltage-independent, non-selective cation channel which is permeable to sodium, potassium, and calcium ions. It is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous system. Three isoforms of NALCN result from alternative splicing events.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 (11568). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody (11568) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NALCN Antibody (D) Composite.
Description Western Blot analysis of Rat Brain showing detection of ~200 kDa NALCN protein using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 (11568). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody (11568) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 (11568). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody (11568) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NALCN Antibody. (D) Composite.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~200kDa.

Positive control: rat brain lysate.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet