Anti-Hsp40 (Ydj1) Antibody (11114)
$384.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | WB,ELISA,IP | Yeast |  |
Overview
Product Name Anti-Hsp40 (Ydj1) Antibody (11114)
Description Anti-HSp40 (Ydj1) Mouse Monoclonal Antibody
Target Hsp40 (Ydj1)
Species Reactivity Yeast
Applications WB,ELISA,IP
Host Mouse
Clonality Monoclonal
Clone ID 1G10.H8
Isotype IgG1
Immunogen Full-length yeast Hsp40 (Ydj1)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes yeast Hsp40 (Ydj1). It does not cross-react with human, mouse, or rat Hsp40.
Target Name Mitochondrial protein import protein MAS5
Target ID Hsp40 (Ydj1)
Uniprot ID P25491
Alternative Names Yeast dnaJ protein 1
Gene Name YDJ1
Gene ID 855661
Accession Number NP_014335.1
Sequence Location Cytoplasm. Cytoplasm, perinuclear region. Note=Concentrated in a perinuclear ring as well as in the cytoplasm.
Biological Function Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex. {PubMed:11689685}.
Research Areas Heat Shock& Stress Proteins
Background Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected
User should should determine optimal concentrations for their application.
User should should determine optimal concentrations for their application.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet