Anti-FKBP52 Antibody (11103)

$479.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP Human, Mouse, Rat, Dog, Hamster
SKU: 11103 Categories: , ,
Overview
Product Name Anti-FKBP52 Antibody (11103)
Description Anti-FKBP52 Mouse Monoclonal Antibody
Target FKBP52
Species Reactivity Human, Mouse, Rat, Dog, Hamster
Applications WB,IHC,ICC/IF,IP
Host Mouse
Clonality Monoclonal
Clone ID Hi52C
Isotype IgG1
Immunogen Recombinant full-length FKBP52 with an N-terminal polyhistidine tag.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, rat, dog, and hamster FKBP52.
Target Name Peptidyl-prolyl cis-trans isomerase FKBP4
Target ID FKBP52
Uniprot ID Q02790
Alternative Names PPIase FKBP4, EC 5.2.1.8, 51 kDa FK506-binding protein, FKBP51, 52 kDa FK506-binding protein, 52 kDa FKBP, FKBP-52, 59 kDa immunophilin, p59, FK506-binding protein 4, FKBP-4, FKBP59, HSP-binding immunophilin, HBI, Immunophilin FKBP52, Rotamase [Cleaved into: Peptidyl-prolyl cis-trans isomerase FKBP4, N-terminally processed]
Gene Name FKBP4
Gene ID 2288
Accession Number NP_002005.1
Sequence Location Cytoplasm, cytosol, Mitochondrion, Nucleus, Cytoplasm, cytoskeleton, Cell projection, axon
Biological Function Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria. {PubMed:1279700, PubMed:1376003, PubMed:19945390, PubMed:21730050, PubMed:2378870}.
Research Areas Heat Shock& Stress Proteins
Background FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Tom Ratajczak, Univ. of W. Australia.
Description Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Tissue: prostate tissue (ductal epithelial cells). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1:1000. Courtesy of: David F. Smith, Mayo Clinic, USA.
Description Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Description Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Description Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/mL. A band of ~52 kDa is detected.

Immunohistochemistry: use at 4-5ug/mL

Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts.
References & Data Sheet
References Nair, SC et al. 1997 Mol Cell Biol 17: 594- 603
PMID 9001212