Overview
Product Name Hsp90 alpha (11102P-5)
Description Recombinant human Hsp90 produced in E. coli is a single, non-glycosylated polypeptide containing 732 amino acids with a molecular weight of 86.8kDa. It is expressed with an N-terminal 6X His-tag
Target Hsp90 alpha
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered colorless solution (1mg/ml) in 20mM Tris-HCl, pH 7.4 and 100mM NaCl.
Buffer Formulation 20 mM Tris
Buffer pH pH 7.4
Molecular Mass Molecular weight of 86.8 kDa
Purity >90% as determined by RP- HPLC and SDS-PAGE
Specificity Information
Target Name Heat shock protein 90kDa alpha (cytosolic), member A1
Target ID Hsp90 alpha
Alternative Names Hsp90alpha
Sequence Location Nucleus , Cytoplasm , Melanosome , Cell membrane , Mitochondrion , Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Sequence MGSSHHHHHH SSGLVPRGSH MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLEDPQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VD
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks of receipt. Store at or below -20°C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long term-storage.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet
Data Sheet Download PDF Data Sheet