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Anti-α B Crystallin Antibody (11073)

$384.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB Human, Mouse, Rat, Bovine, Chicken
SKU: 11073 Categories: , ,
Overview
Product Name Anti-α B Crystallin Antibody (11073)
Description Anti-α B Crystallin Rabbit Polyclonal Antibody
Target α B Crystallin
Species Reactivity Human, Mouse, Rat, Bovine, Chicken
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to human alpha B crystallin.
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Whole Antiserum
Purification Whole antiserum
Specificity Information
Specificity This antibody recognizes human, mouse, rat, bovine, and chicken alpha B crystallin. It does not cross-react with alpha A crystallin.
Target Name α-crystallin B chain
Target ID α B Crystallin
Uniprot ID P02511
Alternative Names α(B-crystallin, Heat shock protein β-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Gene Name CRYAB
Gene ID 1410
Accession Number NP_001276736
Sequence Location Cytoplasm, Nucleus, Secreted, Lysosome
Biological Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity). {UniProtKB:P23927}.
Research Areas Heat Shock& Stress Proteins
Background Alpha crystallins are water-soluble lens proteins of the vertebrate eye that are related to the small heat shock protein family. Lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are further divided into acidic (Alpha A) and basic (Alpha B) groups. In the lens, alpha crystallin maintains proper refractive index, however it can also function as a molecular chaperone that binds to denatured proteins, keeping them in solution and maintaining the translucency of the lens. In response to cellular stress, alpha crystallin is phosphorlyated and may serve a structural control function and play a role in protein maintenance. Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases. Alpha B crystallin is found in many cells and organs outside the lens and is over-expressed in cells subjected to stress conditions and in several neurological disorders.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~22 kDa is detected.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.
References & Data Sheet