Anti-α A Crystallin Antibody (11075)
$384.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Mouse | 100ug | WB,ELISA | Human, Mouse, Rat, Bovine |  |
Overview
Product Name Anti-α A Crystallin Antibody (11075)
Description Anti-α A Crystallin Mouse Monoclonal Antibody
Target α A Crystallin
Species Reactivity Human, Mouse, Rat, Bovine
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 1H3.B8
Isotype IgG1
Immunogen Native alpha crystallin
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, rat, and bovine alpha A crystallin. Other species have not been tested. It does not cross-react with alpha B crystallin, beta-L crystallin, beta- H crystallin, gamma crystallin, Hsp25, Hsp27, or Hsp47.
Target Name α-crystallin A chain
Target ID α A Crystallin
Uniprot ID P02489
Alternative Names Heat shock protein β-4, HspB4 [Cleaved into:α-crystallin A(1-172;α-crystallin A(1-168;α-crystallin A(1-162]
Gene Name CRYAA
Gene ID 1409
Accession Number NP_000385
Sequence Location Cytoplasm, Nucleus
Biological Function Contributes to the transparency and refractive index of the lens (PubMed:18302245). In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions (PubMed:22120592, PubMed:31792453, PubMed:18199971, PubMed:19595763). Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed:28935373). {PubMed:18199971, PubMed:19595763, PubMed:22120592, PubMed:28935373, PubMed:31792453, PubMed:18302245}.
Research Areas Heat Shock& Stress Proteins
Background Alpha crystallins are water-soluble lens proteins of the vertebrate eye that are related to the small heat shock protein family. Lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are further divided into acidic (Alpha A) and basic (Alpha B) groups. In the lens, alpha crystallin maintains proper refractive index, however it can also function as a molecular chaperone that binds to denatured proteins, keeping them in solution and maintaining the translucency of the lens. In response to cellular stress, alpha crystallin is phosphorlyated and may serve a structural control function and play a role in protein maintenance. Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of ~20 kDa is detected.
Positive control: Purified alpha A crystallin.
User should determine optimal concentrations for their application.
Positive control: Purified alpha A crystallin.
User should determine optimal concentrations for their application.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet