Anti-VSV-G Antibody (18876)

$2,415.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 1mg WB,IP,ICC,ELISA Vsv-G
SKU: 18876 Categories: , ,
Overview
Product Name Anti-VSV-G Antibody (18876)
Description Anti-VSV-G Rabbit Polyclonal Antibody
Target VSV-G
Species Reactivity Vsv-G
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID VSV-G
Research Areas Epitope Tags
Background The Vesicular Stomatitis Virus Glycoprotein (VSV-G) epitope tag is represented by the sequence YTDIEMNRLGK. This tag is often included in recombinant proteins to facilitate identification and isolation of those proteins.
Application Images
Description Detection of VSV-G-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-VSV-G antibody 18876 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet
References Tsurumi A et al. 2011. PLoS Genetics 7: e1002086.
Xia F et al. 2008. BloS Biology 6: e128.