Awaiting product image

Anti-Vitronectin Antibody (42025)

$497.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 200ug WB,ELISA,IHC Human
SKU: 42025 Categories: , ,
Overview
Product Name Anti-Vitronectin Antibody (42025)
Description Anti-Human Vitronectin Mouse Monoclonal Antibody
Target Vitronectin
Species Reactivity Human
Applications WB,ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID VN58-1
Isotype IgG1
Immunogen Human Vitronectin
Properties
Form Lyophilized
Formulation 10 mM PBS, 1% BSA, pH 7.4, lyophilized
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody specifically reacts with an epitope in the region of amino acids 1- 130 of human vitronectin. It does not react with bovine vitronectin, and this antibody does not interfere with vitronectin-mediated adhesion.
Target Name Vitronectin
Target ID Vitronectin
Uniprot ID P04004
Alternative Names VN, S-protein, Serum-spreading factor, V75 [Cleaved into: Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B]
Gene Name VTN
Sequence Location Secreted, extracellular space
Biological Function Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.; Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Research Areas Cell adhesion
Application Images
Additional Information
Handling
Storage The stock solution (2. 0mg/ml) can be stored in aliquots at - 20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored.
Dilution Instructions Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).
Application Instructions ELISA: 1:3,000 dilution with solid phase antigen

Western Blot: 5-10 ug/ml under reduced and non-reduced conditions.

Immunohistochemistry: 5-10 ug/ml on paraffin embedded and frozen tissue sections.
References & Data Sheet
References Ang XM et al. Macromolecular Crowding Amplifies Adipogenesis of Human Bone Marrow- Derived Mesenchymal Stem Cellsby Enhancing the Pro-Adipogenic Microenvironment. Tissue Engineering Part A Volume 20: 966, 2014. Western blottingGorur A. et al. COPII-coated membranes function as transport carriers of intracellular procollagen I. J Cell Biol April 20, 2017, PMID 28428367. Immunofluorescence