Anti-VGlut3 Transporter Antibody (56521)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56521 Categories: , ,
Overview
Product Name Anti-VGlut3 Transporter Antibody (56521)
Description Anti-VGlut3 Transporter Mouse Monoclonal Antibody
Target VGlut3 Transporter
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S34-34
Isotype IgG1
Immunogen Fusion protein corresponding to aa 546-588 (cytoplasmic C-terminus) of rat VGlut3 (accession no. NP_74947.1).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat VGlut3. It does not cross-react with VGlut1 or VGlut2.
Target Name Vesicular glutamate transporter 3
Target ID VGlut3 Transporter
Uniprot ID Q7TSF2
Alternative Names VGluT3, Solute carrier family 17 member 8
Gene Name Slc17a8
Sequence Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Membrane, Cell junction, synapse, synaptosome
Biological Function Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {PubMed:12097496, PubMed:12388773}.
Research Areas Neuroscience
Background The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut3 is a multipass membrane protein restricted to synaptic vesicles of glutamatergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT3 Monoclonal Antibody, Clone S34-34 (56521). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT3 Monoclonal Antibody (56521) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT3 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34 (56521). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody (56521) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGlut3 Antibody. (D) Composite.
Additional Information
Handling
Storage This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~65kDa is detected.

Immunohistochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: CV-1 fibroblasts transfected with VGlut3.
References & Data Sheet