Anti-VGlut2 Transporter Antibody (56515)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56515 Categories: , , ,
Overview
Product Name Anti-VGlut2 Transporter Antibody (56515)
Description Anti-VGlut2 Transporter Mouse Monoclonal Antibody
Target VGlut2 Transporter
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S29-29
Isotype IgG1
Immunogen Fusion protein corresponding to aa 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession no. NP_445879).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat VGlut2.
Target Name Vesicular glutamate transporter 2
Target ID VGlut2 Transporter
Uniprot ID Q9JI12
Alternative Names VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6
Gene Name Slc17a6
Sequence Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell junction, synapse, synaptosome
Biological Function Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:11551935, PubMed:11698619, PubMed:11698620, PubMed:11502256, PubMed:17046815, PubMed:18080752). May also mediate the transport of inorganic phosphate (PubMed:17046815). Involved in the regulation of retinal hyaloid vessel regression during postnatal development (By similarity). May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas (PubMed:11551935). {UniProtKB:Q8BLE7, PubMed:11502256, PubMed:11551935, PubMed:11698619, PubMed:11698620, PubMed:17046815, PubMed:18080752}.
Research Areas Neuroscience
Background The ATP-dependent, chloride-sensitive vesicular glutamate transporters include VGlut1, VGlut2, and VGlut3. VGlut1 and VGlut2 are expressed in brain in a complementary fashion. VGlut1 is expressed in the telencephalic regions whereas VGlut2 is expressed in the lower brainstem and diencephalic regions. VGlut3 is expressed in a small number of neurons in the striatum, hippocampus, cerebral cortex, and raphe nuclei. Pancreatic alpha and beta cells express VGlut1 and VGlut2 in response to glucose concentrations.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (56515). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (56515) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT2 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (56515). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (56515) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGLUT2 Antibody. (D) Composite.
Description Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (56515). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (56515) at 1:000.
Additional Information
Handling
Storage This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~60kDa is detected.

Immunohistochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: rat brain lysate.
References & Data Sheet