Anti-Versican Antibody (56542)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF Mouse, Rat
SKU: 56542 Categories: , ,
Overview
Product Name Anti-Versican Antibody (56542)
Description Anti-Versican Mouse Monoclonal Antibody
Target Versican
Species Reactivity Mouse, Rat
Applications WB,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S351-23
Isotype IgG1
Immunogen Fusion protein corresponding to aa 362-585 of mouse Versican (accession no. Q62059).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse and rat Versican.
Target Name Versican core protein
Target ID Versican
Uniprot ID Q62059
Alternative Names Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Large fibroblast proteoglycan, PG-M
Gene Name Vcan
Sequence Location Secreted, extracellular space, extracellular matrix, Cell projection, cilium, photoreceptor outer segment, Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix
Biological Function May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Research Areas Neuroscience
Background Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteo- glycan involved in cell growth and differentiation. Versican contains hyaluronic acid and glycosaminoglycan- binding domains,epidermal growth factor- like repeats, a Lectin-like sequence, and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in atherosclerotic lesions suggests a role for Versican in atherogenesis.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (56542). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (56542) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Versican Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (56542). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (56542) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Versican Antibody. (D) Composite.
Description Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versican protein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (56542). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (56542) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. Predicted molecular weight is >350kDa.

Positive control: Mouse brain lysate.

These are recommended concentrations. User should determine optimal concentrations for their application.
References & Data Sheet