Anti-V5 Antibody (18870)

$2,415.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 1mg WB,IP,ICC,ELISA V5
SKU: 18870 Categories: , ,
Overview
Product Name Anti-V5 Antibody (18870)
Description Anti-V5 Rabbit Polyclonal Antibody
Target V5
Species Reactivity V5
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID V5
Research Areas Epitope Tags
Background The V5 epitope tag represents aa 95-108 (GKPIPNPLLGLDST) derived from RNA polymerase alpha subunit of simian virus 5.
Application Images
Description Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-V5 antibody 18870 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet
References Tsurumi A et al. 2011. PLoS Genetics 7: e1002086
Xia F et al. 2008. BloS Biology 6: e128.