Anti-TRIP230 Antibody (3342)

$503.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC/IF,IP Human
SKU: 3342 Categories: , ,
Overview
Product Name Anti-TRIP230 Antibody (3342)
Description Anti-TRIP230 Mouse Monoclonal Antibody
Target TRIP230
Species Reactivity Human
Applications WB,ICC/IF,IP
Host Mouse
Clonality Monoclonal
Clone ID 10G5
Isotype IgG1
Immunogen GST fusion protein corresponding to aa 1099-1372 of Trip230 expressed in E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human Trip230, a 230 kD protein. The gene for Trip230 is located on chromosome 14q31 in a region associated with genetic alterations and thyroid hormone response abnormalities. Trip230 interacts with Retinoblasoma Protein (Rb) and thyroid hormone receptor using two distinct domains.
Target Name Thyroid receptor-interacting protein 11
Target ID TRIP230
Uniprot ID Q15643
Alternative Names TR-interacting protein 11, TRIP-11, Clonal evolution-related gene on chromosome 14 protein, Golgi-associated microtubule-binding protein 210, GMAP-210, Trip230
Gene Name TRIP11
Gene ID 9321
Accession Number NP_004230
Sequence Location Golgi apparatus, cis-Golgi network membrane, Cytoplasm, cytoskeleton, Endoplasmic reticulum-Golgi intermediate compartment membrane
Biological Function Is a membrane tether required for vesicle tethering to Golgi. Has an essential role in the maintenance of Golgi structure and function (PubMed:25473115, PubMed:30728324). It is required for efficient anterograde and retrograde trafficking in the early secretory pathway, functioning at both the ER-to-Golgi intermediate compartment (ERGIC) and Golgi complex (PubMed:25717001). Binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. {PubMed:10189370, PubMed:25473115, PubMed:25717001, PubMed:30728324, PubMed:9256431}.
Research Areas Cancer Research
Application Images
Description Immunofluorescence analysis of methanol-fixed HeLa, using TRIP230(3342) antibody at 1:100 dilution.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting,

Immunoprecipitation: use at 1-5 ug/mL.

Positive control: MCF7, HBL100, and T24 cells and recombinant fusion protein.
References & Data Sheet
References Chen, Y et al. 1999. PNAS (USA) 96: 4443-4448
Chang, KH et al. 1997. PNAS (USA) 94: 9040-9045.