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Anti-TRICK2 Antibody (11092)

$384.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ELISA Human
SKU: 11092 Categories: , ,
Overview
Product Name Anti-TRICK2 Antibody (11092)
Description Anti-TRICK2 (DR5,TRAIL-R2) Rabbit Polyclonal Antibody
Target TRICK2
Species Reactivity Human
Applications WB,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids 309-325 (EHLLEPAEAERSQRRRL, isoform 1) of human TRICK2 precursor conjugated to KLH at the N-terminus. The same sequence is found in isoform 2 at amino acids 280-296.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human TRICK2.
Target Name Tumor necrosis factor receptor superfamily member 10B
Target ID TRICK2
Uniprot ID O14763
Alternative Names Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, CD antigen CD262
Gene Name TNFRSF10B
Gene ID 8795
Accession Number NP_003833.4
Sequence Location Membrane; Single-pass type I membrane protein.
Biological Function Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. {PubMed:10542098, PubMed:10549288, PubMed:15322075}.
Research Areas Apoptosis
Background Apoptosis is induced by cytokines in the TNF family through their death domain containing receptors. TRICK2 (TRAIL receptor inducer of cell killing 2) is a member of the TNF receptor family and contains the conserved death domain. TRICK2 is expressed at high levels in lymphocytes and spleen cells and in many types of tumor cells. Overexpression of TRICK2 rapidly induces cell death by apoptosis. TRICK2 has been shown to function as a receptor for TRAIL and can inhibit TRAIL-induced killing of lymphocytic cells. Other names for TRICK2 include DR5, Apo2, TRAIL-R2, and KILLER.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500 dilution. A band of approximately 57 kDa is detected.

Positive control: whole cell lysate from HL60, HeLa, or Jurkat cells.
References & Data Sheet
References Screaton, GR et al. (1997) Curr Biol 7: 693-696.
PMID 9285725