Anti-Transglutaminase-2 (TG2) Antibody (90021)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ICC Human, Guinea Pig
SKU: 90021 Categories: , ,
Overview
Product Name Anti-Transglutaminase-2 (TG2) Antibody (90021)
Description Anti-Transglutaminase-2 (TG2) Mouse Monoclonal Antibody
Target Transglutaminase-2 (TG2)
Species Reactivity Human, Guinea Pig
Applications WB,ICC
Host Mouse
Clonality Monoclonal
Clone ID 7D2
Isotype IgG3
Immunogen Purified TG2 from guinea pig liver (accession no. P21980).
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes guinea pig and human TG2.
Target Name Protein-glutamine γ-glutamyltransferase 2
Target ID Transglutaminase-2 (TG2)
Uniprot ID P21980
Alternative Names EC 2.3.2.13, Erythrocyte transglutaminase, Heart Gα(h, hhGα(h, Isopeptidase TGM2, EC 3.4.-.-, Protein Gα(h, G(h, Protein-glutamine deamidase TGM2, EC 3.5.1.44, Protein-glutamine dopaminyltransferase TGM2, EC 2.3.1.-, Protein-glutamine histaminyltransferase TGM2, EC 2.3.1.-, Protein-glutamine noradrenalinyltransferase TGM2, EC 2.3.1.-, Protein-glutamine serotonyltransferase TGM2, EC 2.3.1.-, Tissue transglutaminase, tTG, tTgase, Transglutaminase C, TG(C, TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase II, TGase II, Transglutaminase-2, TG2, TGase-2, hTG2
Gene Name TGM2
Sequence Location Cytoplasm, cytosol, Nucleus, Chromosome, Secreted, extracellular space, extracellular matrix, Cell membrane, Mitochondrion
Biological Function Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively (PubMed:9252372, PubMed:23941696, PubMed:31991788). Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis (PubMed:1683874, PubMed:7935379, PubMed:9252372, PubMed:27270573). Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54 (PubMed:23941696, PubMed:24349085, PubMed:29618516, PubMed:30458214). Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses (PubMed:7649299, PubMed:7592956, PubMed:18092889). When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds (PubMed:12506096). Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components (PubMed:7935379, PubMed:9252372). In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively (PubMed:23797785, PubMed:30867594). Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases (By similarity). Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3 (PubMed:30867594, PubMed:32273471). Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription (PubMed:30867594). Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system (PubMed:32273471). Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2 (By similarity). Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate (PubMed:9623982, PubMed:20547769). Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet (PubMed:9623982). May also act as an isopeptidase cleaving the previously formed cross-links (PubMed:26250429, PubMed:27131890). Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism (PubMed:8943303). {UniProtKB:P08587, UniProtKB:P21981, PubMed:12506096, PubMed:1683874, PubMed:18092889, PubMed:20547769, PubMed:23797785, PubMed:23941696, PubMed:24349085, PubMed:26250429, PubMed:27131890, PubMed:29618516, PubMed:30458214, PubMed:30867594, PubMed:31991788, PubMed:32273471, PubMed:7592956, PubMed:7649299, PubMed:7935379, PubMed:8943303, PubMed:9252372, PubMed:9623982, PubMed:27270573}.; [Isoform 2]: Has cytotoxic activity: is able to induce apoptosis independently of its acyltransferase activity. {PubMed:17116873}.
Research Areas Enzymes
Background Transglutaminase 2 (TG2) is a multi- domain, multi-functional enzyme that post-translationally modifies proteins by catalyzing the formation of inter- molecular isopeptide bonds between glutamine and lysine side-chains. It plays a role in diverse biological functions, including extracellular matrix formation, integrin mediated signaling, and signal transduction involving 7- transmembrane receptors. While some of the roles of TG2 under normal physiological conditions remain obscure, the protein is believed to participate in the pathogenesis of several unrelated diseases including celiac sprue, neurodegenerative diseases, and certain types of cancer.
Application Images
Description Immunoblotting: use at 1-5ug/ml. A band of ~80kDa is detected. Detection of TG2 in human erythroctyes with #90021.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions Immunoblotting: use at 1-5ug/mL. A band of ~80kDa is detected.

Immunocytochemistry: use at 2- 10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet