Anti-Transcription Factor IIB Antibody (70103)

$428.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Mouse	200ug	WB,ELISA,Gel Shift,Inhibition	Human, Mouse

SKU: 70103 Categories: Antibody Products, Products, Protein Transcription Antibodies

Overview

Product Name Anti-Transcription Factor IIB Antibody (70103)

Description Anti-Transcription Factor IIB Mouse Monoclonal Antibody

Target Transcription Factor IIB

Species Reactivity Human, Mouse

Applications WB,ELISA,Gel Shift,Inhibition

Host Mouse

Clonality Monoclonal

Clone ID IIB8

Isotype IgG2a

Immunogen Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by Protein G affinity chromatography

Specificity This antibody recognizes an epitope within amino acids 52-105 of human and mouse TFIIB.

Target Name Transcription initiation factor IIB

Target ID Transcription Factor IIB

Uniprot ID Q00403

Alternative Names EC 2.3.1.48, General transcription factor TFIIB, S300-II

Gene Name GTF2B

Sequence Location Nucleus, Chromosome

Biological Function General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-rPubMed:10318856, PubMed:10619841, PubMed:12931194, PubMed:1517211, PubMed:16230532, PubMed:1876184, PubMed:1946368, PubMed:24441171, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:7675079, PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329}.

Research Areas Protein Transcription

Handling

Storage These antibodies are stable for at least one (1) year at -20°C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions This antibody may be used in ELISA, Western blot, or electrophoretic mobility (gel shift) assays to identify human TFIIB. It can also immuno-deplet TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts. This antibody is polyol-responsive and can be used for gentle purification of TFIIB.

References Thompson NE and Burgess RR. 1994. Protein Expression Purification 5: 468- 475
Thompson NE et al. 1995. J Biol Chem 270: 4735-4740.

PMID 7827504
7533165

Data Sheet