Anti-TATA Binding Protein Antibody (70102)
$441.00
Host | Quantity | Applications | Species Reactivity | Data Sheet | |
---|---|---|---|---|---|
Mouse | 200ug | WB,IP,Gel Shift | Human, Mouse, Rat |
SKU: 70102
Categories: Antibody Products, Products, Protein Transcription Antibodies
Overview
Product Name Anti-TATA Binding Protein Antibody (70102)
Description Anti-TATA - Binding Protein Mouse Monoclonal Antibody
Target TATA Binding Protein
Species Reactivity Human, Mouse, Rat
Applications WB,IP,Gel Shift
Host Mouse
Clonality Monoclonal
Clone ID 1TBP18
Isotype IgG1
Immunogen Recombinant human TBP.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus. Other species not investigated.
Target Name TATA-box-binding protein
Target ID TATA Binding Protein
Uniprot ID P20226
Alternative Names TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit
Gene Name TBP
Sequence Location Nucleus
Biological Function General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. {PubMed:15970593, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:26638071, PubMed:27193682, PubMed:9836642, ECO:0000305}.
Research Areas Protein Transcription
Background The TATA-binding protein (TBP) plays a central role in the assembly of most eukaryotic transcription initiation complexes. TBP assembles with other proteins to form unique multimeric complexes for each of the three different nuclear RNA polymerases. TBP has been cloned from a variety of species and consists of two distinct domains. The C-terminal domain (~180 amino acids) is highly conserved among species, whereas the N-terminal domain varies considerably in length and sequence among different species. The conserved C-terminal domain contains the DNA-binding region as well as regions that interact with positive and negative regulatory proteins. In human TBP, the non-conserved N-terminal domain includes a stretch of glutamine residues ranging from 26 to 42. Several research groups have found an association between expansion of this polyglutamine tract with rare forms of spinocerebellar ataxia and other neurodegenerative diseases including Huntington's disease.
Application Images
Description A. Reactivity with rhuTBP and HeLa cell nuclear extract with MAb #70102 at 2ug/ml. B. Epitope mapping by immunoblot with fragments of rhuTBP with MAB #70102 at 2ug/ml.
Handling
Storage This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions This antibody may be used in Western blot and electrophoretic mobility (gel shift) assays to detect mammalian TBP. It may also be used to immunoprecipitate a truncated TBP molecule containing only the N-terminal domain.
References & Data Sheet
References Thompson NE et al. 2004 Protein Expression and Purification 36: 186-197.
Garcia-Jove Navarro, M., Basset, C., et al. PLoS One . 2013 Aug 7
8(8):e71443
Ribeiro, J. R., Lovasco, L. A., et al. Front Oncol . 2014 Mar 11
4:45
Bouwman, R. D., Palser, A., et al.Retrovirology . 2014 Jul 3
11:53
Leonard, A., Paton, A. W., et al.PLoS One . 2014 Oct 30
9(10):e110949
Kang, K. A., Piao, M. J., et al.Oncotarget. 2016 Jun 28
7(26):40594-40620
Cai, T., Sun, D., et al.J Cell Mol Med. 2018 Mar
22(3):1684-1695
López de la Oliva, A. R., Campos-Sandoval, J. A., et al.Sci Rep. 2020 Feb 10
10(1):2259 Human BLyS facilitates engraftment of human PBL derived B cells in immunodeficient mice. PLoS One (2008) [18784835]
Cytoplasmic CUG RNA foci are insufficient to elicit key DM1 features. PLoS One (2008) [19092997]
PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression. PLoS One (2011) [21390327]
Identification and characterization of peripheral T-cell lymphoma-associated SEREX antigens. PLoS One (2011) [21887344]
Circulating MiR-125b as a marker predicting chemoresistance in breast cancer. PLoS One (2012) [22523546]
Human immunodeficiency virus Tat associates with a specific set of cellular RNAs. Retrovirology (2014) [24990269]
Increased global transcription activity as a mechanism of replication stress in cancer. Nat Commun (2016) [27725641]
FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. Elife (2018) [29809150]
IRF-7 Mediates Type I IFN Responses in Endotoxin-Challenged Mice. Front Immunol (2020) [32373120]
Garcia-Jove Navarro, M., Basset, C., et al. PLoS One . 2013 Aug 7
8(8):e71443
Ribeiro, J. R., Lovasco, L. A., et al. Front Oncol . 2014 Mar 11
4:45
Bouwman, R. D., Palser, A., et al.Retrovirology . 2014 Jul 3
11:53
Leonard, A., Paton, A. W., et al.PLoS One . 2014 Oct 30
9(10):e110949
Kang, K. A., Piao, M. J., et al.Oncotarget. 2016 Jun 28
7(26):40594-40620
Cai, T., Sun, D., et al.J Cell Mol Med. 2018 Mar
22(3):1684-1695
López de la Oliva, A. R., Campos-Sandoval, J. A., et al.Sci Rep. 2020 Feb 10
10(1):2259 Human BLyS facilitates engraftment of human PBL derived B cells in immunodeficient mice. PLoS One (2008) [18784835]
Cytoplasmic CUG RNA foci are insufficient to elicit key DM1 features. PLoS One (2008) [19092997]
PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression. PLoS One (2011) [21390327]
Identification and characterization of peripheral T-cell lymphoma-associated SEREX antigens. PLoS One (2011) [21887344]
Circulating MiR-125b as a marker predicting chemoresistance in breast cancer. PLoS One (2012) [22523546]
Human immunodeficiency virus Tat associates with a specific set of cellular RNAs. Retrovirology (2014) [24990269]
Increased global transcription activity as a mechanism of replication stress in cancer. Nat Commun (2016) [27725641]
FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. Elife (2018) [29809150]
IRF-7 Mediates Type I IFN Responses in Endotoxin-Challenged Mice. Front Immunol (2020) [32373120]
PMID 15249040
23940755
24653979
25356743
27259240
29193729
18784835
19092997
21390327
21887344
22523546
24990269
27725641
29809150
32373120
32042057
23940755
24653979
25356743
27259240
29193729
18784835
19092997
21390327
21887344
22523546
24990269
27725641
29809150
32373120
32042057
Data Sheet Download PDF Data Sheet