Anti-Survivin (mouse CT) Antibody (2237)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P Mouse
SKU: 2237 Categories: , ,
Overview
Product Name Anti-Survivin (mouse CT) Antibody (2237)
Description Anti-mSurvivin (CT) Rabbit Polyclonal Antibody
Target Survivin (mouse CT)
Species Reactivity Mouse
Applications ELISA,WB,IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide corresponding to aa 128-140 of mouse Survivin (accession no. BAA28266).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes mouse Survivin (17kDa).
Target Name Baculoviral IAP repeat-containing protein 5
Target ID Survivin (mouse CT)
Uniprot ID O70201
Alternative Names Apoptosis inhibitor 4, Apoptosis inhibitor survivin, TIAP
Gene Name Birc5
Gene ID 11799
Accession Number NP_001159
Sequence Location Cytoplasm, Nucleus, Chromosome, Chromosome, centromere, Cytoplasm, cytoskeleton, spindle, Chromosome, centromere, kinetochore, Midbody
Biological Function Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:25778398). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (By similarity). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (By similarity). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (By similarity). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (By similarity). May counteract a default induction of apoptosis in G2/M phase (By similarity). The acetylated form represses STAT3 transactivation of target gene promoters (By similarity). May play a role in neoplasia. Inhibitor of CASP3 and CASP7 (By similarity). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). {UniProtKB:O15392, PubMed:25778398}.
Research Areas Apoptosis
Background Apoptosis can be prevented by the inhibitor of apoptosis proteins (IAPs). IAPs constitute a conserved gene family that binds to and inhibits cell death proteases. A novel IAP has been identified and designated Survivin. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is expressed predominantly in tissues of embryos, transformed cell lines, and many human cancers.
Application Images
Description Western blot analysis of survivin in mouse spleen tissue lysate with survivin antibody at 1 ug/mL.
Description Immunohistochemistry of Survivin in mouse spleen cells with Survivin antibody at 10 #956;g/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Positive control: Mouse spleen cell lysate.

Immunohistochemistry: use at 10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet