Anti-Superoxide dismutase (SOD) Mn Antibody (13003)

$479.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IP,ELISA Human, Monkey, Mouse, Rat, Bovine, Canine, Porcine, Hamster, Guinea Pig, Rabbit, Sheep, Chicken, Drosophila, Xenopus
SKU: 13003 Categories: , ,
Overview
Product Name Anti-Superoxide dismutase (SOD) Mn Antibody (13003)
Description Anti-Superoxide dismutase (SOD) Mn Rabbit Polyclonal Antibody
Target Superoxide dismutase (SOD) Mn
Species Reactivity Human, Monkey, Mouse, Rat, Bovine, Canine, Porcine, Hamster, Guinea Pig, Rabbit, Sheep, Chicken, Drosophila, Xenopus
Applications WB,IHC,IP,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Human Mn SOD
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.0, 50% glycerol, and 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.0
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by antigen immunoaffinity chromatography
Specificity Information
Specificity This antibody detects a 25kDa protein on SDS-PAGE immunoblots corresponding to the molecular mass of Mn SOD. This antibody recognizes human, monkey, mouse, rat, bovine, canine, porcine, hamster, guinea pig, rabbit, sheep, chicken, Drosophila, and Xenopus Mn SOD.
Target Name Superoxide dismutase [Mn], mitochondrial
Target ID Superoxide dismutase (SOD) Mn
Uniprot ID P04179
Alternative Names EC 1.15.1.1
Gene Name SOD2
Accession Number NP_000627.2
Sequence Location Mitochondrion matrix.
Biological Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:10334867}.
Research Areas Enzymes
Background Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme that catalyzes the dismutation of the superoxide radical O2- to oxygen and hydrogen peroxide which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. SODs play an important role in antioxidant defense mechanisms. Three different SOD isoenzymes are found in mammalian cells: SOD1, SOD2, and SOD3.
Application Images
Description Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (13003). Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (13003) at 1:100. Left: Untreated, Right: treated with 3mmol*kg-1 NAC. Courtesy of: E. Barreiro, IMIM, Spain.
Description Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD (Mn) protein using Rabbit Anti-SOD (Mn) Polyclonal Antibody (13003). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 µg). Lane 3: Rat Brain (15 µg). Lane 4: Rat Brain Membrane (15 µg). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (13003) at 1:1000 for O/N at 4C. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 24.7kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.2-0.5ug/mL.

Positive control: rat brain tissue extract

Immunohistochemistry: 1-10ug/mL

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet