Anti-Superoxide dismutase (SOD) Cu/Zn Antibody (13002)

$479.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC,IP,ELISA Human, Monkey, Bovine, Mouse, Rat, Canine, Hamster, Pig, Sheep, Rabbit, Xenopus
SKU: 13002 Categories: , ,
Overview
Product Name Anti-Superoxide dismutase (SOD) Cu/Zn Antibody (13002)
Description Anti-Superoxide dismutase (SOD) Cu/Zn Rabbit Polyclonal Antibody
Target Superoxide dismutase (SOD) Cu/Zn
Species Reactivity Human, Monkey, Bovine, Mouse, Rat, Canine, Hamster, Pig, Sheep, Rabbit, Xenopus
Applications WB,IHC,IP,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen Human Cu/Zn SOD Accession no.: P00441, NP_000445.1
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.0, 50% glycerol, 0.09%sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.0
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by antigen immunoaffinity chromatography
Specificity Information
Specificity This antibody detects a 23kDa protein in human (and a19 kDa protein in other species) on SDS-PAGE immunoblots, corresponding to the molecular mass of Cu/Zn SOD. This antibody recognizes human, mouse, rat, canine, porcine, rabbit, ovine, Xenopus, and bovine Cu/Zn SOD.
Target Name Superoxide dismutase [Cu-Zn]
Target ID Superoxide dismutase (SOD) Cu/Zn
Uniprot ID P00441
Alternative Names EC 1.15.1.1, Superoxide dismutase 1, hSod1
Gene Name SOD1
Accession Number NP_000445.1
Sequence Location Cytoplasm, Mitochondrion, Nucleus
Biological Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:24140062}.
Research Areas Enzymes
Background Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme that catalyzes the dismutation of the superoxide radical O2- to oxygen and hydrogen peroxide which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. SODs play an important role in antioxidant defense mechanisms. Three different SOD isoenzymes are found in mammalian cells: SOD1, SOD2, and SOD3.
Application Images
Description Immunohistochemistry analysis using Rabbit Anti-SOD1 Polyclonal Antibody (13002). Tissue: Placenta. Species: Human. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody (13002) at 1:100. Courtesy of: Courtesy of Joan Telfer, University of Glasgow.
Description Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody (13002). Load: 15 µgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody (13002) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL

Immunohistochemistry: use at 1-10ug/mL

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet