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Anti-STAT6 (phospho-Thr645) Antibody (43051)

$366.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human
SKU: 43051 Categories: ,
Overview
Product Name Anti-STAT6 (phospho-Thr645) Antibody (43051)
Description Anti-STAT6 (phospho-Thr645) Rabbit Polyclonal Antibody
Target STAT6 (phospho-Thr645)
Species Reactivity Human
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of threonine 645 (P-A-T(p)-I-K) derived from human STAT6 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human STAT6 only when phosphorylated at threonine 645.
Target Name Signal transducer and activator of transcription 6
Target ID STAT6 (phospho-Thr645)
Uniprot ID P42226
Gene Name STAT6
Gene ID 601512
Post Translational Modification STAT6 (phospho-Thr645)
Target Phospho-thr645
Sequence Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.
Biological Function Carries out a dual signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. {PubMed:17210636}.
Research Areas Phosphospecific Antibodies
Background Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of~110kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
References & Data Sheet