Anti-STAT3 (phospho-Tyr705) Antibody (43045)
$366.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | WB,IHC | Human, Mouse, Rat |  |
Overview
Product Name Anti-STAT3 (phospho-Tyr705) Antibody (43045)
Description Anti-STAT3 (phospho-Tyr705) Rabbit Polyclonal Antibody
Target STAT3 (phospho-Tyr705)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from human STAT3 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat STAT3 only when phosphorylated at tyrosine 705.
Target Name Signal transducer and activator of transcription 3
Target ID STAT3 (phospho-Tyr705)
Uniprot ID P40763
Gene Name STAT3
Gene ID 102582
Post Translational Modification STAT3 (phospho-Tyr705)
Target Phospho-tyr705
Sequence Location Cytoplasm, Nucleus
Biological Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). {UniProtKB:P42227, PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476, PubMed:28065600}.
Research Areas Phosphospecific Antibodies
Background Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~88kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
Immunohistochemistry: use at dilution of 1:50-1:100.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet