Anti-Sphingosine Kinase type 1 Antibody (13810)

$454.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	50ug	IHC,IHC-P	Human

SKU: 13810 Categories: Antibody Products, Enzymes and Enzyme Inhibitor Antibodies, Products

Overview

Product Name Anti-Sphingosine Kinase type 1 Antibody (13810)

Description Anti-Sphingosine Kinase Type 1 Rabbit Polyclonal Antibody

Target Sphingosine Kinase type 1

Species Reactivity Human

Applications IHC,IHC-P

Host Rabbit

Clonality Polyclonal

Immunogen Synthetic peptide conjugated to KLH.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS containing 0.1% sodium azide

Buffer Formulation Phosphate Buffered Saline

Buffer Anti-Microbial 0.1% Sodium Azide

Format Purified

Purification Purified by peptide immuno-affinity chromatography

Specificity This antibody recognizes an epitope within the N-terminal domain of human SPHK1.

Target Name Sphingosine kinase 1

Target ID Sphingosine Kinase type 1

Uniprot ID Q9NYA1

Alternative Names SK 1, SPK 1, EC 2.7.1.91, Acetyltransferase SPHK1, EC 2.3.1.-

Gene Name SPHK1

Accession Number NP_001136073

Sequence Location Cytoplasm, Nucleus, Cell membrane, Endosome membrane, Membrane, clathrin-coated pit, Cell junction, synapse

Biological Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:20577214, PubMed:23602659, PubMed:29662056, PubMed:24929359, PubMed:11923095). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion (PubMed:20577214). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed:23935096). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:28049734, PubMed:24929359). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698). {PubMed:11923095, PubMed:16118219, PubMed:20577214, PubMed:23602659, PubMed:23935096, PubMed:24929359, PubMed:25417698, PubMed:28049734, PubMed:29662056}.; Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. {UniProtKB:Q8CI15}.

Research Areas Enzymes

Background Sphingosine kinase (SPHK) phosphorylates sphingosine to form a bioactive lipid, sphingosine 1-phosphate (S1P). S1P is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival while extracellularly it is a ligand for endothelial differentiation gene 1 (EDG1). Expression of SPHK1 and subsequent production of S1P promotes the G1-S transition, protects cells from apoptosis, stimulates migration and angiogenesis, and induces tumor formation in mice.