Anti-Sodium Iodide Symporter Antibody (11563)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,AM Human, Mouse, Rat
SKU: 11563 Categories: , ,
Overview
Product Name Anti-Sodium Iodide Symporter Antibody (11563)
Description Anti-Sodium Iodine Symporter Mouse Monoclonal Antibody
Target Sodium Iodide Symporter
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF,AM
Host Mouse
Clonality Monoclonal
Clone ID FP5
Isotype IgG1
Immunogen Fusion protein consisting of aa468-643 of human NIS attached to mannose binding protein (MBP).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse and rat NIS.Immunohistochemistry: use at 1-10ug/ml. Positive control: Graves disease-thyroid tissue.These are recommended concentrations; enduser should determine optimal concentrations for their
Target Name Sodium/iodide cotransporter
Target ID Sodium Iodide Symporter
Uniprot ID Q92911
Alternative Names Na(+/I(- cotransporter, Sodium-iodide symporter, Na(+/I(- symporter, Solute carrier family 5 member 5
Gene Name SLC5A5
Accession Number NP_000444.1
Sequence Location Membrane; Multi-pass membrane protein.
Biological Function Mediates iodide uptake in the thyroid gland.
Research Areas Ion Channels
Background The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells. This is an important step in the process of iodide organification and the formation of triiodothyronine and thyroxine. The importance of NIS is related to its role in thyroid function, the pathogenesis of thyroid disease, and its expression (or lack of expression) in thyroid malignancies. It has also been suggested that NIS may represent an autoantigen in autoimmune disease of the thyroid.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Description Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A major band of ~97kDa is detected. Depending on the sample, some minor bands may be detected as well.
References & Data Sheet