Anti-SNAT1 Neurotransmitter Transporter Antibody (56522)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF Human, Mouse, Rat
SKU: 56522 Categories: , ,
Overview
Product Name Anti-SNAT1 Neurotransmitter Transporter Antibody (56522)
Description Anti-SNAT1 Neurotransmitter Transporter Mouse Monoclonal Antibody
Target SNAT1 Neurotransmitter Transporter
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,ICC/IF
Host Mouse
Clonality Monoclonal
Clone ID S104-32
Isotype IgG1
Immunogen Fusion protein corresponding to aa 1-63 (cytoplasmic N-terminus) of rat SNAT1 (accession no. NP_620187.1).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat SNAT1.
Target Name Sodium-coupled neutral amino acid transporter 1
Target ID SNAT1 Neurotransmitter Transporter
Uniprot ID Q9JM15
Alternative Names Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Solute carrier family 38 member 1, System A amino acid transporter 1, System A transporter 2, System N amino acid transporter 1
Gene Name Slc38a1
Sequence Location Cell membrane
Biological Function Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA. {PubMed:10660562, PubMed:11692272, PubMed:11756489, PubMed:12684517}.
Research Areas Neuroscience
Background The sodium-coupled neutral amino acid transporters (SNAT) include System A subtypes SNAT1, SNAT2, and SNAT4 and System N subtypes SNAT3 and SNAT5. These transporters are essential for uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. SNAT1 is responsible for transport of glutamine and may be involved in the generation of glutamate in the retina. SNAT1 is found in heart, brain, and placenta, and expression levels are high in certain neuronal populations in the central nervous system.
Application Images
Description Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (56522). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (56522) at 1:1000.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (56522). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (56522) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (56522). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (56522) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SNAT1 Antibody. (D) Composite.
Additional Information
Handling
Storage This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~50kDa is detected.

Immunohistochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Rat neocortical neurons cultured under amino acid starvation conditions.
References & Data Sheet