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Anti-Ski Antibody (34021)

$400.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IF,IP Human, Mouse, Rat, Chicken, Xenopus
SKU: 34021 Categories: , ,
Overview
Product Name Anti-Ski Antibody (34021)
Description Anti-SKI Mouse Monoclonal Antibody
Target Ski
Species Reactivity Human, Mouse, Rat, Chicken, Xenopus
Applications WB,IF,IP
Host Mouse
Clonality Monoclonal
Clone ID G8
Isotype IgG1
Immunogen Recombinant v-ski
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes ski protein of human, mouse, rat, chicken, and Xenopus.
Target Name Ski oncogene
Target ID Ski
Uniprot ID P12755
Alternative Names Proto-oncogene c-Ski
Gene Name SKI
Sequence Location Nucleus.
Biological Function May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. {PubMed:19049980}.
Research Areas Cancer research
Background The ski oncogene family (ski and sno) encodes transcriptional regulators that can affect oncogenic transformation and cellular differenti- ation. ski and sno function as co- activators or co-repressors, modulating transcription by inter- acting with transcription factor complexes. ski interacts with members of the Nuclear Factor I (NFI) family, the retinoic acid receptor, and the nuclear hormone co-regulators NCoR and NCoA62/skip.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1)year at -70°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-5 ug/ml. Predicted molecular weight of v-ski is 55 kDa; full-length ski ranges from ~95 kDa up to ~115 kDa for hyperphosphorylated ski

Immunocytochemistry: use at 1-10 ug/ml. ski is a nuclear protein.

Immunoprecipitation: use at 1-5 ug/ml.

Positive controls: Recombinant cell lines expressing ski proteins.
References & Data Sheet